Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat CXC-chemokine receptor 4 (also known as CXCR4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat CXCR4 antibody. CXCR4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CXCR4 Antibody is added and binds to CXCR4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CXCR4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat CXCR4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm